biol+4+DNA+manipulation+SAC

This SAC is based on your excursion to CSIRO education centre. You will have the double lesson on Monday 4th August.

You should answer all the relevant questions in the handout prior to Monday's lesson and make sure you have all your experimental results ready. The hand out is NOT being assessed. You will be provided with a range of questions to answer in Monday's lesson.

You will be allowed to bring the handout from that excursion. Make sure you also have a calculator and ruler.

Gene technologies is a large area and you need not worry about the fine detail for all of them. For instance you **do not** need to worry about
 * gene sequencing
 * FISH technique
 * microarrays
 * gene therapy
 * southern blotting

But the following need to be understood well:

//Molecular tools and techniques// restriction enzymes, gel electrophoresis, PCR, recombinant DNA

//Applications// DNA profiling, detecting mutations, transforming bacteria

It is imperative that you are familiar with all the relevant terminology associated with DNA and these techniques. The SAC will contain questions that
 * relate to the experiment procedure, either directly to the excursion or other contexts
 * involve application of theory in a variety of contexts

You can post any questions relating to the SAC below.


 * Q: do we need to know about the application southern blotting???? **

// A. No you do not need to know about this for the SAC. VM //


 * Q. I think i already know the answer to this but im going to ask anyway! Do we need to worry about: cell reproduction, packaging of DNA, DNA replication, gene expression and the different kinds of mutations for the SAC? (that is, the majority of things in the first booklet you gave us)? **

// A. The SAC is not specifically about these things but there is an expectation that you understand the theory behind the applications and techniques we have for manipulating DNA. This means you do need to know about DNA structure and replication gene expression and mutations. VM //